Tim Jeske, Peter Huypens, Laura Stirm, Selina Höckele, Christine Wurmser, Anja Böhm, Cora Weigert, Harald Staiger, Christoph Klein, Johannes Beckers, and Maximilian Hastreiter
The DEUS R package is a framework for accurate small non-coding RNA (sncRNA) profiling based on differential expression of unique sequences. In comparison to commonly-used mapping-based approaches, DEUS generates read counts on the actual sequence reads. Several advantages of this approach include:
- Reads that do not map or map to multiple features are retained in the analysis and represented in an unprecedented and more concise manner
- Sequence clustering provides unique insight into potential processing and editing steps among members of the same cluster
- No need for a reference genome and, therefore, facilitates sncRNA profiling in virtually any organism
- Easy to use and flexible due to high modularity
- Returns intuitively interpretable results